Resources

Calculators

Online Tools

Interactive calculators and models I've developed for the research community:

• Percent solids to particles per mL - Convert percentage solids to particle concentration
• Flow cell model - Model flow cell collection efficiency
• Snells law model - Calculate light refraction angles

Courses

Educational Materials

Course materials and documentation from extracellular vesicle and flow cytometry training programs:

Recent Courses

• 2025 - UVA EV Course [doc]

• 2024 - 47th Annual Flow Course

• 2024 - UVA EV Course [doc, data]

• 2023 - 46th Annual Flow Course

• 2022 - 45th Annual Flow Course

Software

Active Projects

• FCMPASS - Flow cytometry standardization and analysis software
  • Status: Actively maintained
  • Purpose: Standardization of flow cytometry measurements for small particles

Maintained by NIH

• RPSPASS - Reference particle standardization

  • Status: Maintained by NIH
  • *Purpose: Automated Spectradyne calibration and gating software

• MPAPASS - Multiplex particle analysis software

  • Status: Maintained by NIH
  • Purpose: Multiplex bead-based assay analysis

Getting Started

New to EV Research?

If you're new to extracellular vesicle research or flow cytometry, I'd recommend:

1. Start with the MISEV guidelines for standardized EV research
2. Use FCMPASS to calibrate your flow cytometry data
3. Join the small particle flow cytometry discord community for support [join here]

Contact

For questions about any of these resources or to request training materials, please feel free to reach out through the contact information in the footer links.