Last Update: October 2019

Flow cytometry is a highly utilized and incredibly powerful technique owing to it being a high-throughput, high-sensitivity, multi-parametric analysis tool. Flow cytometers have been utilized in research for several decades and as such are very well characterized instruments.

Small particle research is not new on flow cytometers. As early as the late 70s, single viruses were analyzed using flow cytometry. In recent years, extracellular vesicle analysis has been conducted using flow cytometry. While a great deal of research has been done on extracellular vesicles using flow cytometry, the vast majority of literature is not standardized making it impossible to properly interpret or reproduce published data. It has become clear to the field that standardisation is needed for small particle analysis, and much greater knowledge of flow cytometers themselves is required to perform small particle analysis when compared to cellular analysis.

Flow cytometry is my favorite analysis technique, and I have spent my research career to-date studying how it works, how it can be improved, and how it can be standardized.

I have been fortunate enough to disassemble, rebuild, and work with industry in the optimization of flow cytometers. Over time, I aim to use this space to share what I have learned from my research, in the hope it helps others better understand their instruments, learn why it is critical calibrate their instruments, and how to perform fluorescence and light scatter calibration. Where possible this will include interactive learning resources and cite published literature.

Current Material

  1. Why is calibration needed?
  2. Influence of collection angle
  3. How-to guide
  4. Recommended reading