Last Update: October 2019
The use of calibration for any meteorological technique is critical to confirm that measurements are reliable and reproducible. Flow cytometry measurements are focused around the quantitation of light, in the form of light scatter and fluorescence. Flow cytometry data is displayed in arbitrary units which cannot be directly compared between instrument settings or different instruments without first being converted to known (calibrated) units
The calibration of flow cytometer light scatter and fluorescence are both possible, and commercially available reagents and software are available. While not heavily utilized by the cellular analysis field, for whom most flow cytometers are predominantly designed for, the use of calibration is essential for the small particle field where sensitivity is limited and no flow cytometer is capable of detecting the full range of extracellular vesicles.